Organoids and spheroids imaging

Long term imaging of a wide range of organoids

Multi-positions long term imaging (up to weeks)
Easy sample mounting and medium exchange
Wide range of organoid types (intestinal, pancreas, brain, gastruloids)
Formation of luminal structures in brain organoids
Akanksha Jain, group of Barbara Treutlein
D-BSSE Basel
Intestinal organoid development from a single cell
Gustavo de Medeiros, group of Prisca Liberali
FMI Basel

Publications using LS1 Live

Yang et al., Cell fate coordinates mechano-osmotic forces in intestinal crypt morphogenesis. Nature Cell Biology 2021
Rossi et al., Embryonic organoids recapitulate early heart organogenesis. Cell Stem Cell 2020
He et al.: Lineage recording reveals dynamics of cerebral organoid regionalization. bioRvix 2020
Serra et al., Self-organization and symmetry breaking in intestinal organoids development. Nature 2019.

Oocytes and Embryos imaging

Imaging of oocytes and embryos in micro-drop culture

Precise temperature and CO2 and O2 control ensure optimal sample development.
Compatible with micro-drop culture.
High NA detection objective to resolve subcellular structures (e.g. single chromosome / kinetochores).
Mouse embryo development from two cells stage to blastocyst
Diane Pelzer, group of Jean-Leon Maitre
Institut Curie, Paris

Publications using LS1 Live

Dumortier et al.: Fracking and Ostwald ripening position the lumen of the mouse blastocyst. Science 2019
Welling et al.: Primed Track, high-fidelity lineage tracing in mouse pre-implantation embryos using primed conversion of photoconvertible proteins. Elife 2019

Zebrafish and other model organism imaging

Imaging of a variety of model organisms at high resolution

Large field of view up to 800um
Easy multi-positions imaging with no need of agarose embedding
Automatic tracking of region of interest during time-lapse
Dorsal view of Zebrafish somitogenesis
Olivier Venzin, group of Andrew Oates
EPFL Lausanne
Automated online tracking of tail signal in Zebrafish embryo
Arianne Bercowsky, group of Andrew Oates
EPFL Lausanne

Publications using LS1 Live

Rohde et al.: Cell-autonomous generation of the wave pattern within the vertebrate segmentation clock. bioRvix 2021
Naganathan et al.: Somite deformations buffer imprecise segment lengths to ensure left-right symmetry. bioRvix 2020
Arribat et al.: Mitochondria in Embryogenesis: An Organellogenesis Perspective. Frontiers in Cell and Developmental Biology 2019.